Manic Fringe antibody (C-Term)

Catalog No. ABIN6752975

The Rabbit Polyclonal anti-Manic Fringe antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Manic Fringe in samples from Human, Dog, Horse, Guinea Pig, Rabbit and Bat.

Quick Overview for Manic Fringe antibody (C-Term) (ABIN6752975)

Target: Manic Fringe (MFNG) (MFNG)

Reactivity: Human, Dog, Horse, Guinea Pig, Rabbit, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Manic Fringe antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Manic Fringe Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human MFNG

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human MFNG (O00587, NP_002396). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset, Elephant, Panda, Dog, Bat, Rabbit, Horse (100%), Gibbon, Monkey, Galago, Mouse, Rat, Bovine, Opossum, Turkey, Chicken (92%), Hamster, Pig, Guinea pig, Zebra finch, Platypus, Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Manic Fringe

Target: Manic Fringe (MFNG) (MFNG)

Alternative Name: MFNG / Manic Fringe

Background: Name/Gene ID: MFNG

Synonyms: MFNG, Manic fringe

Gene ID: 4242

NCBI Accession: NP_002396

Pathways: Notch Signaling