Retinoblastoma 1 antibody (pSer780) (FITC)

Catalog No. ABIN675301

This Rabbit Polyclonal antibody specifically detects Retinoblastoma 1 in WB, IF (cc), IF (p) and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for Retinoblastoma 1 antibody (pSer780) (FITC) (ABIN675301)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Product Details anti-Retinoblastoma 1 Antibody (FITC)

Binding Specificity: pSer780

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Rb around the phosphorylation site of (Ser780)

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb/p105-Rb

Background:

Synonyms: RbSer780, OSRC, P105 RB, P105RB, PP105, PP110, pRb, RB 1, RB1, RB1 protein, Retinoblastoma 1 including osteosarcoma, Retinoblastoma 1, Retinoblastoma associated protein, Including osteosarcoma, Osteosarcoma, p105-Rb, Rb, RB_HUMAN, Retinoblastoma suspectibility protein, Retinoblastoma-associated protein, Retinoblastoma related osteosarcoma, Retinoblastoma susceptibility gene.

Background: Nuclear Marker.The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Gene ID: 5925

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy