EIF2AK2 antibody (pThr451)

Catalog No. ABIN6757191

The Rabbit Polyclonal anti-EIF2AK2 antibody (ABIN6757191) specifically detects EIF2AK2 in WB, ELISA, IF, ICC and IP. The antibody is reactive with Human samples.

Quick Overview for EIF2AK2 antibody (pThr451) (ABIN6757191)

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-EIF2AK2 Antibody

Binding Specificity: pThr451

Specificity: Detects endogenous levels of human PKR at 74kD. Does not cross-react with other related proteins.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide correspoP9103-74Bnding to residues surrounding Thr451 of human PKR (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, IF, IP (1:100), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry (for Immunofluorescence). Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100. Immunocytochemistry (for IF): 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: EIF2AK2 / PKR

Background: Name/Gene ID: EIF2AK2
Subfamily: Translational Control Kinase
Family: Protein Kinase

Synonyms: EIF2AK2, p1/eIF-2A protein kinase, p68 kinase, PRKR, EIF-2A protein kinase 2, p68, Protein kinase RNA-activated, Tik

Gene ID: 5610

UniProt: P19525

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C