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Leptin antibody (AA 22-167)

This anti-Leptin antibody is a Mouse Monoclonal antibody detecting Leptin in WB. Suitable for Mouse.
Catalog No. ABIN6782282

Quick Overview for Leptin antibody (AA 22-167) (ABIN6782282)

Target

See all Leptin (LEP) Antibodies
Leptin (LEP)

Reactivity

  • 123
  • 74
  • 69
  • 12
  • 8
  • 8
  • 5
  • 5
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  • 4
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  • 2
  • 1
  • 1
Mouse

Host

  • 170
  • 36
  • 7
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  • 1
Mouse

Clonality

  • 183
  • 37
Monoclonal

Conjugate

  • 114
  • 27
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  • 9
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  • 7
  • 3
  • 3
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  • 3
  • 3
  • 3
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  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Leptin antibody is un-conjugated

Application

  • 143
  • 82
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  • 30
  • 18
  • 14
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  • 10
  • 7
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  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 28
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    • 15
    • 15
    • 9
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    • 7
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    • 2
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    • 1
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    AA 22-167

    Specificity

    The antibody is a mouse monoclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.

    Purification

    Protein A + Protein G affinity chromatography

    Immunogen

    Recombinant LEP (Val22-Cys167) expressed in E. coli.

    Type of Immunogen: Recombinant protein

    Isotype

    IgG
  • Application Notes

    Approved: WB (1:100 - 1:400)

    Usage: ELISA 0.05-2 μg/mL, ICC 5-30 μg/mL, WB 0.5-5 μg/mL,

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.02 % sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Store frozen product at or below -20°C. Thawed product may be stored for 2-4 weeks at 4°C. For optimal storage, aliquot to smaller portions and store at -20°C to -70°C. Avoid freeze-thaw cycles.
  • Target

    Leptin (LEP)

    Alternative Name

    Leptin

    Background

    Name/Gene ID: LEP

    Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB

    Gene ID

    3952

    Pathways

    JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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