EPO antibody (AA 101-193)

Catalog No. ABIN679718

The Rabbit Polyclonal anti-EPO antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) and DB. It is suitable to detect EPO in samples from Human and Rat. There are 4+ publications available.

Quick Overview for EPO antibody (AA 101-193) (ABIN679718)

Target: EPO (Erythropoietin (EPO))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Product Details anti-EPO Antibody

Binding Specificity: AA 101-193

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Erythropoietin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
dot-blot()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-EPO antibody (ABIN679718)

Desharnais, Naud, Ayotte: "Detection of erythropoiesis stimulating agents in urine samples using a capillary Western system." in: Drug testing and analysis, Vol. 10, Issue 11-12, pp. 1698-1707, (2019) (PubMed).

Ichii, Nakamura, Irie, Kouguchi, Nakamura, Nakamura, Sato, Yokoyama, Horino, Sunden, Elewa, Kon: "Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes." in: Histochemistry and cell biology, Vol. 146, Issue 3, pp. 351-62, (2016) (PubMed).

Li, Chen, Shao, Tang, Chen, Chen, Xu: "Oxidative stress induces the decline of brain EPO expression in aging rats." in: Experimental gerontology, Vol. 83, pp. 89-93, (2016) (PubMed).

DeNiro, Al-Mohanna: "Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment." in: PLoS ONE, Vol. 9, Issue 7, pp. e101602, (2014) (PubMed).

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO

Target Type: Hormone

Background:

Synonyms: EP, MVCD2, Erythropoietin, EPO

Background: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Gene ID: 2056

UniProt: P01588

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity