PDE5A antibody (AA 101-200)

Catalog No. ABIN679793

The Rabbit Polyclonal anti-PDE5A antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect PDE5A in samples from Human and Rat.

Quick Overview for PDE5A antibody (AA 101-200) (ABIN679793)

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PDE5A Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PDE5A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PDE5A

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Alternative Name: PDE5A

Background:

Synonyms: CN5A, PDE5, CGB-PDE, cGMP-specific 3',5'-cyclic phosphodiesterase, cGMP-binding cGMP-specific phosphodiesterase, PDE5A

Background: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP (PubMed:9714779, PubMed:15489334). Specifically regulates nitric-oxide-generated cGMP (PubMed:15489334).

Gene ID: 8654

UniProt: O76074

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling