Caspase 9 antibody (pThr125)

Catalog No. ABIN683863

This Rabbit Polyclonal antibody specifically detects Caspase 9 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for Caspase 9 antibody (pThr125) (ABIN683863)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Caspase 9 Antibody

Binding Specificity: pThr125

Specificity: This phosphorylation site is homologous to that of Thr163 in Mouse.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Caspase-9 around the phosphorylation site of Thr125

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Caspase 9 antibody (ABIN683863)

Bozok Cetintas, Acikgoz, Yigitturk, Demir, Oktem, Tezcanli Kaymaz, Oltulu, Aktug: "Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells." in: Medicine, Vol. 95, Issue 43, pp. e5150, (2016) (PubMed).

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Caspase-9

Background:

Synonyms: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, CASP9

Background: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Gene ID: 842

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity