FOXO1 antibody (pThr24, pThr32)

Catalog No. ABIN684673

This Rabbit Polyclonal antibody specifically detects FOXO1 in WB, ELISA, IF (p), IHC (p), IF (cc) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for FOXO1 antibody (pThr24, pThr32) (ABIN684673)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FOXO1 Antibody

Binding Specificity: pThr24, pThr32

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human FOXO1 around the phosphorylation site of Thr24+Thr32

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FOXO1/3/4+

Background:

Synonyms: FOXO4 phospho T32, p-FoxO1 Thr24/FoxO3a Thr32, FOXO3A phospho T32, p-FOXO3A phospho T32, Forkhead box protein O1, Afxh, AI876417, FKHR, Fkhr1, Foxo1a, Forkhead, FKH 1, FKH1, FKH1, FKHR, FKHR, Forkhead Drosophila homolog 1 rhabdomyosarcoma, Forkhead Drosophila homolog 1 rhabdomyosarcoma, Forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, Forkhead, Drosophila, homolog of, in rhabdomyosarcoma, FOXO1, FOXO1, FOXO1_HUMAN, FOXO1A, forkhead box protein O3.

Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined, however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq].

Gene ID: 2308

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling