FOXO1 antibody (pSer256)

Catalog No. ABIN684748

This Rabbit Polyclonal antibody specifically detects FOXO1 in WB, ELISA, IF (p), IHC (p), IF (cc), FACS and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Cow and has been mentioned in 2+ publications.

Quick Overview for FOXO1 antibody (pSer256) (ABIN684748)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FOXO1 Antibody

Binding Specificity: pSer256

Cross-Reactivity: Cow, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human FOXO1 around the phosphorylation site of Ser256 [AA(p-S)MD]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FOXO1 antibody (ABIN684748)

Li, Qiu, Liu, Wang, Hu, Gan, Wang: "Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica)." in: Journal of thermal biology, Vol. 70, Issue Pt B, pp. 37-45, (2017) (PubMed).

Kinoshita, Locher, Tienken, Meyer, Dänicke, Rehage, Huber: "Associations between Forkhead Box O1 (FoxO1) Expression and Indicators of Hepatic Glucose Production in Transition Dairy Cows Supplemented with Dietary Nicotinic Acid." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146670, (2016) (PubMed).

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: Foxo1

Background:

Synonyms: FOXO1A phospho S256, p-FOXO1A phospho S256, FKHRSer256, Forkhead box protein O1, Afxh, AI876417, FKHR, Fkhr1, Foxo1a, Forkhead, FKH 1, FKH1, FKH1, FKHR, FKHR, Forkhead Drosophila homolog 1 rhabdomyosarcoma, Forkhead Drosophila homolog 1 rhabdomyosarcoma, Forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, Forkhead, Drosophila, homolog of, in rhabdomyosarcoma, FOXO1, FOXO1, FOXO1_HUMAN, FOXO1A.

Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined, however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq].

Gene ID: 2308

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling