GRB2 antibody (Cy5)

Catalog No. ABIN686936

The Rabbit Polyclonal anti-GRB2 antibody is suitable to detect GRB2 in samples from Human, Mouse, Rat, Dog and Chicken. It has been validated for IF (p).

Quick Overview for GRB2 antibody (Cy5) (ABIN686936)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB2 antibody is conjugated to Cy5

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-GRB2 Antibody (Cy5)

Specificity: Excitation / Emission Wavelengths: 650nm/670nm

Cross-Reactivity: Rat (Rattus), Chicken, Dog (Canine)

Purification: purified by Protein A

Immunogen: KLH conjugated synthetic peptide derived from human GRB2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P)(1:100-500)
Optimal working dilution should be determined by the investigator.

Comment:

Excitation / Emission wavelength: 652nm / 670nm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 µg/µL

Buffer: Aqueous buffered solution containing 100 µg/mL BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4 °C for 12 months

Expiry Date: 12 months

Target Details for GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Alternative Name: GRB2/ASH

Background: Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.

Molecular Weight: 25kDa

Gene ID: 2885

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation