ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1B (ABCB1B) (AA 1051-1280) antibody

Catalog No. ABIN686977

This Rabbit Polyclonal antibody specifically detects ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1B in ELISA, WB, FACS, IF (cc), IF (p), IHC (fro) and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1B (ABCB1B) (AA 1051-1280) antibody (ABIN686977)

Target: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1B (ABCB1B)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 1051-1280

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MDR1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Mo, Pospichalova, Huang, Murphy, Payne, Wang, Kennedy, Cianciolo, Bryja, Pizzo, Bachelder: "Ascites Increases Expression/Function of Multidrug Resistance Proteins in Ovarian Cancer Cells." in: PLoS ONE, Vol. 10, Issue 7, pp. e0131579, (2015) (PubMed).

Target Details

Target: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1B (ABCB1B)

Alternative Name: MDR1

Background:

Synonyms: P-Glycoprotein, Multi Drug Resistance Associated Protein, ABCB1, ATP-binding cassette sub-family B MDR/TAP, member 1, ABC20, CD243, CD243 antigen, CLCS, Multidrug resistance protein 1, P glycoprotein 1, p-GP, gp170, P gp, PGY1.

Background: P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.

Gene ID: 5243