SMAD3 antibody (pSer423, pSer425) (Biotin)

Catalog No. ABIN6870632

The Biotin-conjugated Rabbit Monoclonal anti-SMAD3 antibody (Clone ST0493) (ABIN6870632) specifically detects SMAD3 in WB, IHC, IF and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for SMAD3 antibody (pSer423, pSer425) (Biotin) (ABIN6870632)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: ST0493

Product Details anti-SMAD3 Antibody (Biotin)

Binding Specificity: pSer423, pSer425

Purpose: Conjugated Smad3(Phospho-S423/S425) Rabbit mAb

Purification: Protein A affinity purified

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser423 and 425 of human Smad3.

Application Details

Application Notes: Biotin conjugated: Working with enzyme-conjugated streptavidin. ELISA: 1: 200 - 1: 1,000; Western Blot: 1: 200 - 1: 1,000; IHC/ICC: 1: 100 - 1: 500; Flow Cytometry: 1: 50 - 1: 250; IF: 1: 100 - 1: 500.

Restrictions: For Research Use only

Handling

Buffer: Liquid in PBS, 0.5 % BSA, 40 % Glycerol, 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light!

Storage: 4 °C

Storage Comment: Store at 4°C in dark for 6 months

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: Smad3

Background: DKFZP586N0721 antibody DKFZp686J10186 antibody hMAD 3 antibody hMAD-3 antibody hSMAD3 antibody HSPC193 antibody HST17436 antibody JV15 2 antibody JV15-2 antibody JV152 antibody LDS1C antibody LDS3 antibody MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody MAD homolog 3 antibody Mad homolog JV15 2 antibody Mad protein homolog antibody MAD, mothers against decapentaplegic homolog 3 antibody Mad3 antibody MADH 3 antibody MADH3 antibody MGC60396 antibody Mothers against decapentaplegic homolog 3 antibody Mothers against DPP homolog 3 antibody SMA and MAD related protein 3 antibody SMAD 3 antibody SMAD antibody SMAD family member 3 antibody SMAD, mothers against DPP homolog 3 antibody Smad3 antibody SMAD3_HUMAN antibody

Molecular Weight: 55 kDa

Gene ID: 4088

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy