ACPP antibody
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- Target See all ACPP Antibodies
- ACPP (Acid Phosphatase, Prostate (ACPP))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ACPP antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Recognizes a protein of 52 kDa, identified as prostate specific acid phosphatase (PSAP). This enzyme catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland.PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Prostatic acid phosphatase Purified from human seminal plasma
- Clone
- PASE-4LJ
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product ACPP Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: PC12 cells. Normal prostate or prostate carcinoma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- ACPP (Acid Phosphatase, Prostate (ACPP))
- Alternative Name
- ACPP (ACPP Products)
- Synonyms
- 5'-NT antibody, ACP-3 antibody, ACP3 antibody, PAP antibody, AMAP2 antibody, CENTB3 antibody, DDEF2 antibody, PAG3 antibody, Pap-alpha antibody, SHAG1 antibody, A030005E02Rik antibody, FRAP antibody, Lap antibody, Ppal antibody, Acpp11 antibody, RNACPP11 antibody, pap antibody, ACPP antibody, acp-3 antibody, acp3 antibody, acpt antibody, acid phosphatase, prostate antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 antibody, prostatic acid phosphatase antibody, prostatic acid phosphatase, putative antibody, acid phosphatase, prostate S homeolog antibody, ACPP antibody, ASAP2 antibody, Acpp antibody, CpipJ_CPIJ004002 antibody, Smp_016640 antibody, acpp.S antibody
- Background
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5'-nucleotidase (5'-NT), Acid phosphatase prostate, ACP3, Ecto-5'-nucleotidase, Prostatic acid phosphatase (PAP), Prostatic acid phosphatase, Thiamine monophosphatase (TMPase),Prostate Specific Acid Phosphatase (PSAP)
Cellular localisation: Cytoplasmic - Molecular Weight
- 52kDa
- Gene ID
- 55, 433060
- UniProt
- P15309
- Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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