ACTA1/ACTA2A/ACTG2 antibody

Catalog No. ABIN6936350

This Mouse Monoclonal antibody specifically detects ACTA1/ACTA2A/ACTG2 in IHC, FACS and IF. It exhibits reactivity toward Human, Rabbit, Rat, Cat, Dog, Chicken and Mouse.

Quick Overview for ACTA1/ACTA2A/ACTG2 antibody (ABIN6936350)

Target: ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))

Reactivity: Human, Rabbit, Rat, Cat, Dog, Chicken, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTA1/ACTA2A/ACTG2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HHF35

Product Details anti-ACTA1/ACTA2A/ACTG2 Antibody

Purpose: Actin, Muscle Specific (Muscle Cell Marker) Antibody

Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Cross-Reactivity (Details): Human. Rabbit. Cat. Dog. Mouse. Rat. Chicken.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: SDS extract of human myocardium.

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Muscle or sarcoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ACTA1/ACTA2A/ACTG2

Target: ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))

Background:

ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1

Muscle or sarcoma.

Gene ID: 58, 59, 70

UniProt: P62736, P68032, P68133