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ACTA1/ACTA2A/ACTG2 antibody

Reactivity: Human IHC (f), WB Host: Mouse Monoclonal MSA-953 unconjugated
Catalog No. ABIN6936352
  • Target See all ACTA1/ACTA2A/ACTG2 products
    ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
    Reactivity
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 4
    Mouse
    Clonality
    • 4
    Monoclonal
    Conjugate
    • 4
    This ACTA1/ACTA2A/ACTG2 antibody is un-conjugated
    Application
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
    Specificity
    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
    Cross-Reactivity (Details)
    Human, Rabbit and Rat.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Synthetic peptide common among human muscle actins
    Clone
    MSA-953
    Isotype
    IgG1 kappa
  • Application Notes

    Known_Application: Western Blot (0.5-1.0 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: Muscle or sarcoma.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Preservative
    Azide free
    Storage
    -20 °C,-80 °C
    Storage Comment
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Expiry Date
    24 months
  • Target
    ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
    Abstract
    ACTA1/ACTA2A/ACTG2 Products
    Background
    ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1,Actin, Muscle Specific (Muscle Cell Marker)
    Cellular localisation: Cytoplasm.
    Molecular Weight
    Multiple
    Gene ID
    58, 59, 70
    UniProt
    P62736, P68032, P68133
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