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ACTA1/ACTA2A/ACTG2 antibody

Reactivity: Human, Rabbit, Rat, Cat, Chicken, Dog, Mouse FACS, IF, IHC, StM Host: Mouse Monoclonal HHF35 unconjugated
Catalog No. ABIN6941339
  • Target See all ACTA1/ACTA2A/ACTG2 products
    ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
    Reactivity
    Human, Rabbit, Rat, Cat, Chicken, Dog, Mouse
    Host
    • 4
    Mouse
    Clonality
    • 4
    Monoclonal
    Conjugate
    • 4
    This ACTA1/ACTA2A/ACTG2 antibody is un-conjugated
    Application
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificity
    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
    Purification
    Purified by Protein A/G
    Immunogen
    SDS extract of human myocardium.
    Clone
    HHF35
    Isotype
    IgG1 kappa
  • Application Notes

    Positive Control: Muscle or sarcoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
    Abstract
    ACTA1/ACTA2A/ACTG2 Products
    Gene ID
    58, 59, 70
    UniProt
    P62736, P68032, P68133
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