ACTA1/ACTA2A/ACTG2 antibody
Quick Overview for ACTA1/ACTA2A/ACTG2 antibody (ABIN6941339)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Actin, Muscle Specific (Muscle Cell Marker) Antibody
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Specificity
- This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
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Purification
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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Immunogen
- SDS extract of human myocardium.
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Isotype
- IgG1, kappa
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anti-ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth) antibody
Reactivity: Human, Rabbit, Rat IHC, WB Host: Mouse Monoclonal MSA-953 unconjugated
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Application Notes
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Positive Control: Muscle or sarcoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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Restrictions
- For Research Use only
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Concentration
- 200 μg/mL
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Buffer
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Expiry Date
- 24 months
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- ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
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Background
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ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1
Muscle or sarcoma.
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Gene ID
- 58, 59, 70
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UniProt
- P62736, P68032, P68133
Target
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