ARF1 antibody (C-Term)

Catalog No. ABIN6936427

The Mouse Monoclonal anti-ARF1 antibody (Clone 3F1) (ABIN6936427) specifically detects ARF1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for ARF1 antibody (C-Term) (ABIN6936427)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 3F1

Product Details anti-ARF1 Antibody

Binding Specificity: AA 174-180, C-Term

Purpose: ARF1 (Golgi Apparatus Marker) Antibody

Specificity: The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1.

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Kidney, Liver, Brain or Breast.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background:

ADP-ribosylation factor 1, ARF1

Brain or Breast., HeLa cells. Kidney, Liver

Molecular Weight: 20kDa

Gene ID: 375, 286221

UniProt: P84077

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process