Calnexin antibody (N-Term)

Catalog No. ABIN6936576

The Mouse Monoclonal anti-Calnexin antibody is suitable to detect Calnexin in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for Calnexin antibody (N-Term) (ABIN6936576)

Target: Calnexin (CANX)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: CANX-1541

Product Details anti-Calnexin Antibody

Binding Specificity: AA 1-300, N-Term

Purpose: Calnexin (Endoplasmic Reticulum Marker) Antibody

Specificity: It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Human. Does not react with Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: CANX

Background:

Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90

HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Molecular Weight: ~90kDa

Gene ID: 821, 567968

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis