Cyclin B2 antibody
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- Target See all Cyclin B2 (CCNB2) Antibodies
- Cyclin B2 (CCNB2)
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Reactivity
- Xenopus laevis
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Cyclin B2 antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC), Coating (Coat)
- Specificity
- In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.
- Cross-Reactivity (Details)
- Mouse. Rat. Human. Xenopus laevis.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length Xenopus laevis Cyclin B2 protein
- Clone
- X29-2
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CCNB2 Primary Antibody
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- Application Notes
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Known_Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Human cervical tissue (IHC).
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Cyclin B2 (CCNB2)
- Alternative Name
- CCNB2 (CCNB2 Products)
- Synonyms
- HsT17299 antibody, CycB2 antibody, sb:cb303 antibody, cycB2 antibody, LOC100136691 antibody, C-B2 antibody, cyclinb2 antibody, hst17299 antibody, LOC100282653 antibody, cyclin B2 antibody, cyclin B2 S homeolog antibody, CCNB2 antibody, Ccnb2 antibody, ccnb2 antibody, LOC100136691 antibody, LOC100282653 antibody, ccnb2.S antibody
- Background
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Ccnb2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, HsT17299, MGC108931, MGC140694,Cyclin B2
Cellular localisation: Cytoplasm. - Molecular Weight
- 51kDa
- Gene ID
- 9133, 194698
- UniProt
- O95067
- Pathways
- Cell Division Cycle, M Phase
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