Cyclin E1 antibody (AA 10-176)

Catalog No. ABIN6936595

The Mouse Monoclonal anti-Cyclin E1 antibody (Clone CCNE1-2587) (ABIN6936595) specifically detects Cyclin E1 in ELISA. The antibody is reactive with Human samples.

Quick Overview for Cyclin E1 antibody (AA 10-176) (ABIN6936595)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: ELISA

Clone: CCNE1-2587

Product Details anti-Cyclin E1 Antibody

Binding Specificity: AA 10-176

Purpose: Cyclin E (G1/S-Phase Cyclin) Antibody

Specificity: Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Breast or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1

Background:

CCNE1, Cyclin E1, G1/S-specific cyclin-E1

HeLa cells. Breast or Colon Carcinoma.

Molecular Weight: 53kDa.

Gene ID: 898, 244723

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases