CD4 antibody

Catalog No. ABIN6936701

This Rat Monoclonal antibody specifically detects CD4 in FACS, IF and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for CD4 antibody (ABIN6936701)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Clone: GK1-5

Product Details anti-CD4 Antibody

Purpose: CD4, Mouse (T-Cell Marker) Antibody

Specificity: CD4 is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally.This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

No Cross-Reactivity: Human

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Mouse CTL clone V4

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: Spleen, Thymus or Lymph Node.

Known Application: ELISA (For coating, order Ab without BSA & Azide), Neutralization Studies (Order Ab without BSA & Azide), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background:

CD4 (L3T4), CD4, CD4+ Lymphocyte deficiency, included, Leu3, Ly-4, Lymphocyte antigen CD4, p55, T cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Spleen, Thymus or Lymph Node.

Molecular Weight: 55kDa

Gene ID: 12504, 2209

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events