CD4 antibody

Catalog No. ABIN6936701

This Rat Monoclonal antibody specifically detects CD4 in FACS, IF, ELISA, Neut and Coat. It exhibits reactivity toward Mouse.

Quick Overview for CD4 antibody (ABIN6936701)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Neutralization (Neut), Coating (Coat)

Clone: GK1-5

Product Details anti-CD4 Antibody

No Cross-Reactivity: Human

Purification: Purified by Protein A/G

Immunogen: Mouse CTL clone V4

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: Spleen, Thymus or Lymph Node.

Known Application: ELISA (For coating, order Ab without BSA & Azide), Neutralization Studies (Order Ab without BSA & Azide), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally.This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Molecular Weight: 55kDa

Gene ID: 12504

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events