CD47 antibody

Catalog No. ABIN6936738

The Mouse Monoclonal anti-CD47 antibody has been validated for FACS, WB, IF and Func. It is suitable to detect CD47 in samples from Human.

Quick Overview for CD47 antibody (ABIN6936738)

Target: CD47

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Functional Studies (Func)

Clone: B6H12-2

Product Details anti-CD47 Antibody

Specificity: This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Intact CD47 Purified from placenta

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Functional Studies (Order Ab without Azide), Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, MCF-7 or OVCA3 cells. Human placenta, brain or ovarian tumors.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen,CD47 / IAP (Integrin Associated Protein)
Cellular localisation: Cell Surface

Molecular Weight: ~50kDa

Gene ID: 961, 446414

UniProt: Q08722