CD53 antibody

Catalog No. ABIN6936756

Product Details anti-CD53 Antibody

Target: CD53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD53 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Immunomicroscopy (IM)

Specificity: Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI, Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

No Cross-Reactivity: Baboon, Horse (Equine)

Cross-Reactivity (Details): Human. Does not react with Baboon or Horse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Stimulated human leukocytes

Clone: 161-2

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Functional Studies (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD53

Target: CD53

Alternative Name: CD53 (CD53 Products)

Synonyms: AI323659 antibody, Ox-44 antibody, Tspan25 antibody, MOX44 antibody, TSPAN25 antibody, OX44 antibody, CD53 antigen antibody, CD53 molecule antibody, Cd53 molecule antibody, Cd53 antibody, CD53 antibody

Background: CD53 glycoprotein, CD53 tetraspan antigen, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, Tetraspanin-25 (Tspan-25), TSPAN25,CD53 (TSPAN25)
Cellular localisation: Cell Surface

Molecular Weight: 33-55kDa

Gene ID: 963, 443057

UniProt: P19397