CYP3A1 antibody

Catalog No. ABIN6937072

Product Details anti-CYP3A1 Antibody

Target: CYP3A1 (Cyp3a1) (Cytochrome P450, Family 3, Subfamily A, Polypeptide 1 (Cyp3a1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYP3A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat), Flow Cytometry (FACS)

Specificity: Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies, the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene. The CYP2 family are part of the microsomal drug metabolising system that is responsible for oxidation of many therapeutic agents as well as steroids, fatty acids and many other endogenous substances. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. P6 is highly specific for members of the CYP 3A gene family and cross reacts with CY 3A4 in human, CYP 3A in rat and potentially CYP 3A11 (based on inducibility) in mice.

Cross-Reactivity (Details): Human. Mouse. Rat. Pig.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full length protein (Rat). Rat liver cytochrome P450 3A1.

Clone: P6

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), ,Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Liver or Cardiac muscle.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CYP3A1

Target: CYP3A1 (Cyp3a1) (Cytochrome P450, Family 3, Subfamily A, Polypeptide 1 (Cyp3a1))

Alternative Name: CYP3A1 (Cyp3a1 Products)

Synonyms: CYP antibody, CYP3A23 antibody, Cyp3a1 antibody, Cyp3a3 antibody, RL33 antibody, cDEX antibody, cytochrome P450, family 3, subfamily a, polypeptide 23/polypeptide 1 antibody, cytochrome P450 3A1 antibody, cytochrome P450, family 3, subfamily a, polypeptide 1 antibody, Cyp3a23/3a1 antibody, VDBG_02397 antibody, Cyp3a1 antibody

Background: CYP3A1, Cytochrome P450 family 3 subfamily A polypeptide 1,Cytochrome P450 3A1 / CYP3A1
Cellular localisation: Endoplasmic reticulum, membrane bound.

Molecular Weight: 51kDa