Desmoglein 1 antibody (Intracellular)
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- Target See all Desmoglein 1 (DSG1) Antibodies
- Desmoglein 1 (DSG1)
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Binding Specificity
- Intracellular
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Desmoglein 1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
- Specificity
- Recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Human desmoglein-1 intracellular domain
- Clone
- 18D4
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product DSG1 Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL) Optimal dilution for a specific application should be determined.
Positive_Control: A431 or A-375 cells. Skin.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Desmoglein 1 (DSG1)
- Alternative Name
- DSG1 (DSG1 Products)
- Synonyms
- CDHF4 antibody, DG1 antibody, DSG antibody, PPKS1 antibody, SPPK1 antibody, DSG-1 antibody, DSG1 antibody, Dsg1b antibody, Dsg1c antibody, desmoglein 1 antibody, desmoglein 4 antibody, DSG1 antibody, DSG4 antibody, Dsg1 antibody
- Background
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Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1,Desmoglein-1 (DSG1)
Cellular localisation: Cell surface - Molecular Weight
- 150kDa (precursor), 160kDa (mature)
- Gene ID
- 1828, 2633
- UniProt
- Q02413
- Pathways
- Cell-Cell Junction Organization
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