ERCC1 antibody (AA 191-281)

Catalog No. ABIN6937182

The Mouse Monoclonal anti-ERCC1 antibody is suitable to detect ERCC1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for ERCC1 antibody (AA 191-281) (ABIN6937182)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: ERCC1-2318

Product Details anti-ERCC1 Antibody

Binding Specificity: AA 191-281

Purpose: ERCC1 / RAD10 (Tumor Progression Marker) Antibody

Specificity: Recognizes a protein of 110 kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 191-281) of human ERCC1 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells. Tonsil or Prostate.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1

Background:

COFS4, DNA excision repair protein ERCC1, ERCC1, Excision repair cross complementing 1, RAD10, UV20

HeLa or HepG2 cells. Human tonsil or prostate.

Molecular Weight: 110kDa

Gene ID: 2067, 435981

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response