CD15 antibody

Catalog No. ABIN6937284

The Rabbit Polyclonal anti-CD15 antibody has been validated for FACS, IF and IHC (f). It is suitable to detect CD15 in samples from Human.

Quick Overview for CD15 antibody (ABIN6937284)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CD15 Antibody

Specificity: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified human neutrophils

Isotype: IgG

Application Details

Application Notes:

Known_Application: Flow Cytometry (2-4ug/million cells); Immunofluorescence (2-4ug/ml); Immunohistochemistry (Formalin-fixed) (5-10ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: FUT4

Background: 3 Fucosyl N Acetyl Lactosamine, Alpha (1,3) Fucosyltransferase, Alpha 13 fucosyltransferase FucT, ELAM Ligand Fucosyltransferase, ELFT, FCT3A, Fuc-TIV, Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific, Fucosyltransferase 4, Galactoside 3 L Fucosyltransferase, Lewis X, LeX, SSEA1, Stage Specific Embryonic Antigen 1,CD15 / FUT4 (Reed-Sternberg Cell Marker)
Cellular localisation: Cell surface and granular paranuclear

Molecular Weight: ~220kDa

Gene ID: 2526, 654379

UniProt: P22083