Recombinant GFAP antibody

Catalog No. ABIN6937313

Product Details anti-GFAP Antibody

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Cross-Reactivity (Details): Human. Mouse. Rat. Cow. Pig. Rabbit. Chicken. Dog

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length GFAP protein

Clone: ASTRO-1974R

Isotype: IgG

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (2-3 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP (GFAP Products)

Synonyms: GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody

Background: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP),GFAP (Astrocyte & Neural Stem Cell Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: ~50kDa

Gene ID: 2670, 514227

UniProt: P14136