HLA-B antibody

Catalog No. ABIN6937396

Product Details anti-HLA-B Antibody

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Normal human peripheral blood lymphocytes

Clone: JOAN-1

Isotype: IgG3 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Human peripheral blood lymphocytes, tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HLA-B

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Alternative Name: HLA-B (HLA-B Products)

Synonyms: AS antibody, HLAB antibody, SPDA1 antibody, mhc1b antibody, major histocompatibility complex, class I, B antibody, major histocompatibility complex nonclassical class Ib L homeolog antibody, HLA-B antibody, BOLA-B antibody, MAMU-B18 antibody, DKFZP469N1225 antibody, mhc1b.L antibody

Background: HLA-B, HLAB, MHC Class I HLA heavy chain, Leukocyte antigen class I-B,HLA-B (MHC Class I)
Cellular localisation: Cell Surface

Molecular Weight: 45kDa

Gene ID: 3106, 654404, 77961

UniProt: P01889

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints