HLA-B antibody

Catalog No. ABIN6937396

The Mouse Monoclonal anti-HLA-B antibody (Clone JOAN-1) (ABIN6937396) specifically detects HLA-B in FACS, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for HLA-B antibody (ABIN6937396)

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: JOAN-1

Product Details anti-HLA-B Antibody

Purpose: HLA-B (MHC Class I) Antibody

Specificity: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Normal human peripheral blood lymphocytes

Isotype: IgG3, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Human peripheral blood lymphocytes, tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HLA-B

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Alternative Name: HLA-B

Background:

HLA-B, HLAB, MHC Class I HLA heavy chain, Leukocyte antigen class I-B

Human peripheral blood lymphocytes, tonsil or lymph node.

Molecular Weight: 45kDa

Gene ID: 3106, 654404, 77961

UniProt: P01889

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response