HLA-DRB1 antibody

Catalog No. ABIN6937415

This Mouse Monoclonal antibody specifically detects HLA-DRB1 in FACS and IF. It exhibits reactivity toward Human, Monkey, Baboon, Chimpanzee, Dog and Squirrel.

Quick Overview for HLA-DRB1 antibody (ABIN6937415)

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Reactivity: Human, Monkey, Baboon, Chimpanzee, Dog, Squirrel

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRB1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: L243

Product Details anti-HLA-DRB1 Antibody

Purpose: HLA-DRB (MHC II) Antibody

Specificity: This MAb reacts with the HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. It binds a conformational epitope on HLA-DR, which depends on the correct folding of the heterodimer. This MAb has been reported to block mixed lymphocyte reactions. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kD alpha (heavy) chain and a 28kD beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Cross-Reactivity (Details): Human. Baboon. Chimpanzee. Monkey. Squirrel. Dog.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Activated human peripheral blood mononuclear cells

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HLA-DRB1

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Alternative Name: HLA-DRB1

Background:

DRB1, HLA class II histocompatibility antigen, DR-1 beta chain, HLA-DR-beta 1, HLA-DRB1, human leucocyte antigen DRB1, Leucocyte antigen DR beta 1 chain, lymphocyte antigen DRB1, major histocompatibility complex, class II, DR beta 1, MHC class II HLA-DR beta 1 chain, MHC class II HLA-DR-beta cell surface glycoprotein

Daudi or HuT78 cells. Tonsil or lymph node., Ramos

Molecular Weight: ~28kDa (beta chain)

Gene ID: 3123, 534322

UniProt: P01911

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response