HLA-DRB3 antibody

Catalog No. ABIN6937422

The Mouse Monoclonal anti-HLA-DRB3 antibody is suitable to detect HLA-DRB3 in samples from Human. It has been validated for IF and FACS.

Quick Overview for HLA-DRB3 antibody (ABIN6937422)

Target: HLA-DRB3 (Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRB3 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: Bra30

Product Details anti-HLA-DRB3 Antibody

Specificity: This MAb reacts with the HLA-DRB3 antigen, a member of MHC class II molecules.It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Cross-Reactivity (Details): Human. Baboon. Chimpanzee. Monkey. Squirrel. Dog.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human REH cells (non-T, non-B leukemia cell line)

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HLA-DRB3

Target: HLA-DRB3 (Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3))

Alternative Name: HLA-DRB3

Background: HLA class II histocompatibility antigen DR beta 3 chain, HLADR3B, Major histocompatibility complex, class II DR beta 3,HLA-DRB (MHC II)
Cellular localisation: Cell Surface

Molecular Weight: ~28kDa (beta chain)

Gene ID: 3123, 534322

UniProt: P01911

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response