Recombinant Insulin Receptor antibody (Extracellular Domain)

Catalog No. ABIN6937575

The Rabbit Monoclonal anti-Insulin Receptor antibody has been validated for IHC (f). It is suitable to detect Insulin Receptor in samples from Human.

Quick Overview for Recombinant Insulin Receptor antibody (Extracellular Domain) (ABIN6937575)

Target: Insulin Receptor (INSR)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: INSR-2277R

Product Details anti-Insulin Receptor Antibody

Binding Specificity: Extracellular Domain

Specificity: The insulin receptor (INSR) is a heterodimeric protein complex that has an intracellular subunit, which is disulfide-linked to a transmembrane segment. The insulin ligand binds to the INSR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to INSR induces phosphorylation of intra-cellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Type 1 diabetes is an autoimmune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of extracellular domain of human Insulin Receptor alpha (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells. Human pancreas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR

Background: CD220, HHF5, HIR A, INSR, Insulin receptor, Insulin receptor subunit alpha, IR,Insulin Receptor Alpha
Cellular localisation: Cytoplasm.

Molecular Weight: 135kDa

Gene ID: 3643, 465744

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity