CD11b antibody

Catalog No. ABIN6937588

The Rat Monoclonal anti-CD11b antibody (Clone M1-70) (ABIN6937588) specifically detects CD11b in FACS, IF and Func. The antibody is reactive with Mouse samples.

Quick Overview for CD11b antibody (ABIN6937588)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: M1-70

Product Details anti-CD11b Antibody

Specificity: CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin aM combines with the Integrin 2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b andCD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.

Cross-Reactivity (Details): Human. Chimpanzee. Baboon. Cynomolgus. Rhesus. Rabbit. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: B10 Mouse spleen cells enriched for T lymphocytes

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Functional Studies (Order Ab without BSA & Azide), Optimal dilution for a specific application should be determined.

Positive_Control: Monocytes & granulocytes. Lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: ITGAM

Background: CD11 antigen-like family member B, CD11b/CD18, CD49d, Cell surface glycoprotein MAC-1 subunit alpha, Complement Component Receptor 3 Alpha, CR3 Alpha Chain (CR3A), Integrin alpha-M, Integrin beta 2 alpha subunit, ITGAM, Leukocyte adhesion receptor MO1, Ly-40, Mac-1a, MAC1, Mac1, alpha subunit, MAC1A, Macrophage antigen alpha polypeptide, MO1A, Neutrophil adherence receptor alpha M subunit,CD11b / MAC-1 (Microglial Marker)
Cellular localisation: Cell Surface

Molecular Weight: 95kDa & 170kDa

Gene ID: 3684, 172631

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation