CD11b antibody

Catalog No. ABIN6937588

The Rat Monoclonal anti-CD11b antibody (Clone M1-70) (ABIN6937588) specifically detects CD11b in FACS, IF and Func. The antibody is reactive with Human, Mouse, Rabbit, Baboon, Rhesus Monkey, Chimpanzee and Cynomolgus samples.

Quick Overview for CD11b antibody (ABIN6937588)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Rabbit, Baboon, Rhesus Monkey, Chimpanzee, Cynomolgus

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: M1-70

Product Details anti-CD11b Antibody

Purpose: CD11b / MAC-1 (Microglial Marker) Antibody

Specificity: CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin aM combines with the Integrin 2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b andCD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.

Cross-Reactivity (Details): Human. Chimpanzee. Baboon. Cynomolgus. Rhesus. Rabbit. Mouse.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: B10 Mouse spleen cells enriched for T lymphocytes

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Functional Studies (Order Ab without BSA & Azide), Optimal dilution for a specific application should be determined.

Positive_Control: Monocytes & granulocytes. Lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: ITGAM

Background:

CD11 antigen-like family member B; CD11b/CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; ITGAM; Leukocyte adhesion receptor MO1; Ly-40; Mac-1a; MAC1; Mac1, alpha subunit; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit

Monocytes & granulocytes. Lymph nodes and tonsils.

Molecular Weight: 95kDa & 170kDa

Gene ID: 3684, 172631

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation