KRT6A antibody

Catalog No. ABIN6937715

Product Details anti-KRT6A Antibody

Target: KRT6A (Keratin 6A (KRT6A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT6A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6A (KRT6A). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns. Cytokeratin 6A is the dominant form in epithelial tissue. Cytokeratin 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Cytokeratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express KRT6 and KRT16. KRT6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas..

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Cytokeratin 6A (KRT6A) protein

Clone: KRT6A-2368

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells, Tonsil or Basal Cell Carcinoma (BCC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for KRT6A

Target: KRT6A (Keratin 6A (KRT6A))

Alternative Name: KRT6A (KRT6A Products)

Synonyms: CK6A antibody, CK6C antibody, CK6D antibody, K6A antibody, K6C antibody, K6D antibody, KRT6C antibody, KRT6D antibody, 2310016L08Rik antibody, Krt2-6a antibody, Krt2-6c antibody, MK6a antibody, mK6[a] antibody, CK-6A antibody, Krt6a antibody, KRT6A antibody, keratin 6A antibody, similar to keratin complex 2, basic, gene 6a antibody, keratin, type II cytoskeletal 6A antibody, KRT6A antibody, Krt6a antibody, LOC683313 antibody, LOC101112304 antibody

Background: 58 kDa Cytokeratin, CK5, Cytokeratin-5, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, KRT5, Type-II Cytoskeletal 5, Type-II keratin Kb5,Cytokeratin 6A (KRT6A) (Basal Cell Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 56kDa

Gene ID: 3853, 700779

UniProt: P02538