MCM6 antibody (AA 228-368)

Catalog No. ABIN6937817

This Mouse Monoclonal antibody specifically detects MCM6 in IHC and IHC (f). It exhibits reactivity toward Human.

Quick Overview for MCM6 antibody (AA 228-368) (ABIN6937817)

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: MCM6-2999

Product Details anti-MCM6 Antibody

Binding Specificity: AA 228-368

Specificity: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 228-368) of human MCM6 protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human skin or squamous cell carcinoma (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MCM6

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Alternative Name: MCM6

Background: DNA replication licensing factor MCM6, MCM6 minichromosome maintenance deficient 6, MIS5 homolog, p105MCM,MCM6 (Proliferation Marker)
Cellular localisation: Nuclear

Molecular Weight: 105kDa

Gene ID: 4175, 444118

UniProt: Q14566

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA