NME2 antibody
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- Target See all NME2 Antibodies
- NME2 (Nucleoside diphosphate kinase B (NME2))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NME2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human NME2 protein
- Clone
- CPTC-NME2-2
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product NME2 Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: PC3, A549, HeLa, Jurkat cells. Ubiquitously expressed in all tissues.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- NME2 (Nucleoside diphosphate kinase B (NME2))
- Alternative Name
- NME2 (NME2 Products)
- Synonyms
- NDKB antibody, NDPK-B antibody, NDPKB antibody, NM23-H2 antibody, NM23B antibody, PUF antibody, nm23-2 antibody, p18-12d antibody, nm23-M2 antibody, NM23-C2 antibody, NME/NM23 nucleoside diphosphate kinase 2 antibody, non-metastatic cells 2, protein (NM23B) expressed in antibody, NME2 antibody, Nme2 antibody
- Background
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C myc purine binding transcription factor PUF, C-myc purine-binding transcription factor PUF, epididymis secretory sperm binding protein Li 155an, HEL-S-155an, Histidine protein kinase NDKB, NDP kinase B, NDPKB, nm23-H2, NM23B, NME/NM23 nucleoside diphosphate kinase 2, nme2, Nucleoside diphosphate kinase B,NME2 / nm23-H2 / NDPK-B (Suppressor of Metastasis)
Cellular localisation: Cytoplasmic and Nuclear - Molecular Weight
- 17kDa
- Gene ID
- 4831, 463456
- UniProt
- P22392
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