NADPH Oxidase 4 antibody

Catalog No. ABIN6938080

The Mouse Monoclonal anti-NADPH Oxidase 4 antibody (Clone NOX4-1245) (ABIN6938080) specifically detects NADPH Oxidase 4 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for NADPH Oxidase 4 antibody (ABIN6938080)

Target: NADPH Oxidase 4 (NOX4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NADPH Oxidase 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: NOX4-1245

Product Details anti-NADPH Oxidase 4 Antibody

Purpose: NOX4 / NADPH Oxidase 4 Antibody

Specificity: The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human NOX4 protein

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA)Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: PC-12, JAR cells. Kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Alternative Name: NOX4

Background:

Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX-1, NADPH oxidase 4, Nox4, Renal NAD(P)H-oxidase, RENOX

JAR cells. Kidney., PC-12

Molecular Weight: 70kDa

Gene ID: 50507, 371036

UniProt: Q9NPH5

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration