OGG1 antibody

Catalog No. ABIN6938109

The Mouse Monoclonal anti-OGG1 antibody is suitable to detect OGG1 in samples from Human. It has been validated for WB and IHC.

Quick Overview for OGG1 antibody (ABIN6938109)

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OGG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CPTC-OGG1-1

Product Details anti-OGG1 Antibody

Purpose: 8-Oxoguanine DNA Glycosylase Antibody

Specificity: 8-oxoguanine (8-oxoG), an oxidized form of guanine, is produced by reactive oxygen species in both DNA and nucleotide pools during normal aging. Accumulation of 8-oxoG increases the occurrence of A:T to C:G or G:C to T:A transversionmutations, because 8-oxoG forms a stable basepair with adenine as well as with cytosine. OGG1 (for 8-oxoG DNA glycosylase), also designated MMH, is a DNA repair enzyme that corrects these mutations. Inactivation of the OGG1 gene leads to a mutator phenotype, characterized by the increase in G:C to T:A transversions. The OGG1 gene encodes eight isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria. Guanine is the main target for reactive oxygen species in DNA, and 8-oxoguanine is the most frequent base lesion. Therefore, formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. Furthermore, defects in OGG1 may be a cause of renal cell carcinoma.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human OGG1 protein

Isotype: IgG2c, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells. Kidney, skin or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for OGG1

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Alternative Name: OGG1

Background:

HMMH, HOGG1, MUTM, OGH1

HeLa or Jurkat cells. Kidney, skin or lymph node, Daudi, MDA-MB-231

Molecular Weight: 39kDa

Gene ID: 4968, 380271

UniProt: O15527

Pathways: DNA Damage Repair