PAPPA antibody (AA 351-523)

Catalog No. ABIN6938112

Product Details anti-PAPPA Antibody

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Binding Specificity: AA 351-523

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Pregnancy Associated Plasma Protein (PAPP-A) is found in maternal blood that increases as pregnancy progresses, although it is not specific to pregnancy. It is principally expressed in the syncytiotrophoblast of the placenta, which forms the main source of circulating maternal PAPP-A. It cleaves insulin-like growth factor binding proteins (IGFBPs), IGFBP-4 and IGFBP-5. IGFBP-4 cleavage is enhanced significantly in the presence of bound IGF, whereas IGFBP-5 cleavage is inhibited slightly by IGF presence. It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. PAPPA has also been suggested as a potential biomarker of acute myocardial infarction and Coronary Artery Disease (CAD).

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (within aa 351-523) of human PAPP-A protein (exact sequence is proprietary)

Clone: PAPPA-2715

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Placenta.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: PAPPA (PAPPA Products)

Synonyms: 8430414N03Rik antibody, IGFBP-4ase antibody, PAG1 antibody, PAPP-A antibody, ASBABP2 antibody, DIPLA1 antibody, PAPA antibody, PAPPA1 antibody, Pappa antibody, pappalysin 1 antibody, pregnancy-associated plasma protein A antibody, PAPPA antibody, Pappa antibody, Pappa1 antibody

Background: ASBABP2, Aspecific BCL2 ARE binding protein 2, Differentially placenta 1 expressed protein, DIPLA1, IGF-dependent, IGFBP4ase, Insulin-like growth factor-dependent IGF-binding protein 4 protease (IGFBP-4 protease), PAPA, PAPP A, PAPPA1, Pappalysin-1, Pregnancy Associated Plasma Protein A (PAPP-A),PAPP-A / Pappalysin-1 (Marker of Atherosclerosis and Aneuploid Fetus)
Cellular localisation: Secreted

Molecular Weight: 181kDa

Gene ID: 5069, 643599

UniProt: Q13219