PAX6 antibody (N-Term)

Catalog No. ABIN6938122

The Mouse Monoclonal anti-PAX6 antibody (Clone PAX6-1166) (ABIN6938122) specifically detects PAX6 in IHC. The antibody is reactive with Human samples.

Quick Overview for PAX6 antibody (N-Term) (ABIN6938122)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: PAX6-1166

Product Details anti-PAX6 Antibody

Binding Specificity: AA 1-300, N-Term

Purpose: PAX6 (Stem Cell Marker) Antibody

Specificity: Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters' anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (N-terminus, aa 1-300) of human PAX6 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Pancreas, Cerebellum or Stomach.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: PAX6

Background:

AN2, Aniridia type II protein, Oculorhombin, Paired box gene 6 (aniridia keratitis), Paired box homeotic gene 6, Paired box protein Pax-6, Sey, WAGR

Cerebellum or Stomach., Pancreas

Molecular Weight: 47kDa

Gene ID: 5080, 611376, 33870

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis