PDLIM1 antibody
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- Target See all PDLIM1 Antibodies
- PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PDLIM1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88 % sequence similarity with its rat counterpart, suggesting a conserved function between species.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human full-length PDLIM1 protein
- Clone
- CPTC-PDLIM1-1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PDLIM1 Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 cells and extracts from Jurkat. Human heart, colon or spleen.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))
- Alternative Name
- PDLIM1 (PDLIM1 Products)
- Synonyms
- CLIM1 antibody, CLP-36 antibody, CLP36 antibody, hCLIM1 antibody, pdlim1 antibody, MGC76041 antibody, MGC64465 antibody, clim1 antibody, wu:fa19a03 antibody, wu:fy34f10 antibody, zgc:110681 antibody, Clim1 antibody, mClim1 antibody, PDLIM1 antibody, PDZ and LIM domain 1 antibody, PDZ and LIM domain 1 L homeolog antibody, PDZ and LIM domain 1 (elfin) antibody, PDLIM1 antibody, Pdlim1 antibody, pdlim1 antibody, pdlim1.L antibody
- Background
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C terminal LIM domain protein 1, carboxy terminal LIM domain protein 1, CLIM1, CLP36, Elfin, Enigma antibody, Enigma homolog antibody, hCLIM1, PDLIM1, PDZ and LIM domain 1,PDZ and LIM Domain 1
Cellular localisation: Cytoplasm. - Molecular Weight
- 36kDa
- Gene ID
- 9124, 368525
- UniProt
- O00151
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