PIP antibody (AA 41-146)

Catalog No. ABIN6938187

Product Details anti-PIP Antibody

Target: PIP (Prolactin-Induced Protein (PIP))

Binding Specificity: AA 41-146

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: It recognizes a protein of 15 kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50 % of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human GCDFP-15 protein fragment (around aa 41-146) (exact sequence is proprietary)

Clone: PIP-1571

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 cells. Breast or Pancreas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PIP

Target: PIP (Prolactin-Induced Protein (PIP))

Alternative Name: PIP (PIP Products)

Synonyms: GCDFP-15 antibody, GCDFP15 antibody, GPIP4 antibody, GP17 antibody, mSMGP antibody, prolactin induced protein antibody, PIP antibody, Pip antibody

Background: BRST-2, GCDFP-15, gp17, GPIP4, Gross cystic disease fluid protein 15, Prolactin-induced protein (PIP), Prolactin-inducible protein (PIP), Secretory actin-binding protein (SABP),GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker)
Cellular localisation: Cytoplasmic (Golgi bodies) and Secreted

Molecular Weight: 15kDa

Gene ID: 5304, 99949

UniProt: P12273