Recombinant Retinoblastoma 1 antibody

Catalog No. ABIN6938312

This Rabbit Monoclonal antibody specifically detects Retinoblastoma 1 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant Retinoblastoma 1 antibody (ABIN6938312)

Target: Retinoblastoma 1 (RB1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RB1-2313R

Product Details anti-Retinoblastoma 1 Antibody

Specificity: Recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Cross-Reactivity (Details): Human. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length Rb1 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: RB1

Background: OSRC, Osteosarcoma, p105-Rb, PP105, pp110, pRb, Prepro retinoblastoma associated protein, RB1, Retinoblastoma 1, Retinoblastoma-associated protein,Rb1 (Tumor Suppressor Protein)
Cellular localisation: Nuclear

Molecular Weight: 105kDa

Gene ID: 5925, 408528

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy