EpCAM antibody

Catalog No. ABIN6938492

This Mouse Monoclonal antibody specifically detects EpCAM in WB, FACS, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for EpCAM antibody (ABIN6938492)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: Cocktail PAN-EpCAM

Product Details anti-EpCAM Antibody

Purpose: EpCAM / CD326 (Epithelial Marker) Antibody

Specificity: It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM). It is a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or EpCAM. EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Abs purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human EpCAM protein (full-length and fragments)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or HT29 cells. Human breast tumor.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: TACSTD1

Background:

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TACSTD1, TROP1, Tumor-associated Calcium Signal Transducer 1

MCF-7 or HT29 cells. Human breast tumor.

Molecular Weight: 40-43kDa

Gene ID: 4072, 542050

UniProt: P16422