TNFRSF4 antibody (AA 59-205)

Catalog No. ABIN6938616

The Mouse Monoclonal anti-TNFRSF4 antibody is suitable to detect TNFRSF4 in samples from Human and Chimpanzee. It has been validated for FACS, ELISA, IHC and IF.

Quick Overview for TNFRSF4 antibody (AA 59-205) (ABIN6938616)

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Reactivity: Human, Chimpanzee

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF4 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: OX40-3108

Product Details anti-TNFRSF4 Antibody

Binding Specificity: AA 59-205

Purpose: OX40 / CD134/ TNFRSF4 (Immuno-Oncology Target) Antibody

Specificity: ThisMAb recognizes a protein of 43 kDa, identified as OX40, which is also known as CD134.OX40 is a type I integral membrane glycoprotein and member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. It is expressed on activated T lymphocytes, hematopoietic precursor cells and fibroblasts. It functions as a T cell co-stimulatory receptor when bound by OX40 Ligand/TNFSF4 that is expressed by antigen presenting cells. OX40 thereby plays roles in T-cell activation as well as the regulation of differentiation, proliferation or apoptosis of normal and malignant lymphoid cells.OX40 is upregulated at the sites of inflammation, especially in case of multiple sclerosis and psoriatic lesions.

Cross-Reactivity (Details): Human. Chimpanzee.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human OX40 (CD134) protein (around aa 59-205) (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4 cells. Human peripheral blood leukocytes (hPBL). Human tonsil tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TNFRSF4

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Alternative Name: TNFRSF4

Background:

ACT35, CD134, Lymphoid activation antigen ACT35, OX40, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4 (Tumor necrosis factor receptor superfamily member 4)

MOLT-4 cells. Human peripheral blood leukocytes (hPBL). Human tonsil tissue (IHC).

Molecular Weight: 43kDa

Gene ID: 7293, 129780

UniProt: P43489

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints