TOP1MT antibody

Catalog No. ABIN6938632

Product Details anti-TOP1MT Antibody

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOP1MT antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human TOP1MT protein

Clone: TOP1MT-568

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Heart, Skeletal muscle, brain or fetal liver.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Alternative Name: TOP1MT (TOP1MT Products)

Synonyms: 2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody

Background: DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase,Topoisomerase (DNA) I, Mitochondrial (TOP1MT)
Cellular localisation: Cytoplasmic (Mitochondria)

Molecular Weight: 70kDa

Gene ID: 116447, 528574

UniProt: Q969P6