Recombinant TOP1MT antibody

Catalog No. ABIN7862180

This Mouse Monoclonal antibody specifically detects TOP1MT in IHC, WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant TOP1MT antibody (ABIN7862180)

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOP1MT antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RTOP1MT-488

Product Details anti-TOP1MT Antibody

Purpose: Recombinant Topoisomerase I, Mitochondrial (TOP1MT) Antibody

Specificity: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human TOP1MT protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Alternative Name: Topoisomerase I, Mitochondrial

Background:

DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase

brain or fetal liver tissue., MCF-7 or A431 cells. Jurkat cell lysate. Human heart, Skeletal muscle

Molecular Weight: 70kDa

Gene ID: 116447, 528574

UniProt: Q969P6