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TOP1MT antibody

TOP1MT Reactivity: Human IF, WB, FACS, IHC (f) Host: Mouse Monoclonal TOP1MT-488 unconjugated
Catalog No. ABIN6938633
  • Target See all TOP1MT Antibodies
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    Reactivity
    • 28
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 19
    • 11
    Mouse
    Clonality
    • 17
    • 13
    Monoclonal
    Conjugate
    • 14
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TOP1MT antibody is un-conjugated
    Application
    • 10
    • 9
    • 9
    • 9
    • 7
    • 4
    • 3
    • 2
    Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificity
    DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
    Cross-Reactivity (Details)
    Human.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Recombinant full-length human TOP1MT protein
    Clone
    TOP1MT-488
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product TOP1MT Primary Antibody
  • Application Notes

    Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: MCF-7 or A431 cells. Jurkat cell lysate. Human heart, skeletal muscle, brain or fetal liver tissue.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Preservative
    Azide free
    Storage
    -20 °C,-80 °C
    Storage Comment
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Expiry Date
    24 months
  • Target
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    Alternative Name
    TOP1MT (TOP1MT Products)
    Synonyms
    2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody
    Background
    DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase,Topoisomerase I, Mitochondrial (TOP1MT)
    Cellular localisation: Cytoplasm (Mitochondria)
    Molecular Weight
    70kDa
    Gene ID
    116447, 528574
    UniProt
    Q969P6
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