CKB antibody

Catalog No. ABIN6939143

This anti-CKB antibody is a Mouse Monoclonal antibody detecting CKB in ELISA, IF, FACS, IHC (fro) and Coat. Suitable for Human.

Quick Overview for CKB antibody (ABIN6939143)

Target: CKB (Creatine Kinase, Brain (CKB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CKB antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Coating (Coat)

Clone: 2ba6

Product Details anti-CKB Antibody

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The specificity of this monoclonal antibody to its intended target was validated by HuProt Array containing more than 19,000 full-length, correctly-folded human proteins. Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins.

Cross-Reactivity (Details): Predicted to react with Chimpanzee, Rhesus Monkey, Dog, Cow, Mouse, Rat, Chicken, Zebrafish, Frog.

Purification: Purified by Protein A/G

Immunogen: Human CKBB protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Cerebellum.

Known Application: ELISA (For coating, order Ab without BSA),Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Frozen Only) (0.5-1.0 μg/mL for 30 minutes at RT) Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CKB

Target: CKB (Creatine Kinase, Brain (CKB))

Alternative Name: CKB

Molecular Weight: 43kDa (Monomer), 86kDa (Dimer)

Gene ID: 1152

UniProt: P12277